棉花晋A及其保持系酯酶过氧化物酶PAGE电泳分析

PAGE Analysis on Esterase and Peroxidase of "JinA-line and B-line" in Cotton

采用聚丙烯酰胺凝胶电泳(PAGE)方法,对晋A棉花质核雄性不育系及其保持系作了酯酶过氧化物酶同工酶分析。结果表明,晋A及其保持系两种同工酶在所有器官上均具有一些共同的基本酶带,在营养器官上无较大差别,说明它们具有基本一致的遗传背景。受育性基因的影响,进入生殖阶段,在造孢细胞增殖时期的花蕾上,晋A较其保持系明显缺少特征谱带,表明在此阶段雄性不育基因调控了同工酶的合成,导致花粉粒发生败育。

The PAGE analysis on JinA male sterile and its B-line of cotton showed that there were some responsible bands of esterase and peroxidase for all tissues, and there were less differences in the same nutrient tissue part between them,which indicated that they had similar genetic background. Obvious differences were found due to the influence of male sterile genes, when entering reproduction stage, two isozymes of JinA were obvious less than that of B-line in ″sporophyte-cell multiplication″ period ,which showed that the male sterile genes controlled the synthesis of esterase and peroxidase isozymes ,so the abortive pollens were informed.