甘蓝型油菜辐照新不育系Mx1与Mx2及其保持系线粒体DNA的RAPD分析

RAPD Analysis on Mitochondrial DNA of the Cytoplasmic Male Sterile Lines Mx1 and Mx2 Induced by Radiation and Its Maintainer

Mx1和Mx2是通过辐射甘蓝型油菜瑞典品种得到的细胞质雄性不育系。采用RAPD技术对Mx1和Mx2及其保持系陕2B的线粒体DNA(mtDNA)进行了比较研究。共使用了210个随机引物,其中有163个引物在两系之间都得到了扩增产物,15个引物扩增结果在两系之间表现出了遗传多态性。Mx1和Mx2间也存在着明显的差异。

Mx1 and Mx2 were two cytoplasmic male sterile lines which was obtained from Swedish variety of Brassica napus irradiated by gamma ray. A comparative analysis for mitochondrial DNAs (mtDNAs) between Mx1 and Mx2 and their maintainer were performed by using RAPD techniques. There were 163 primers giving amplified products in two lines.Among them 15 primers presented polymorphism. There was significant difference between Mx1 and Mx2 in RAPD amplified fragments.