利用RAPD标记研究燕麦属不同种的遗传差异

Genetic Diversity Revealed by RAPD in Different Species of Avena

利用RAPD标记对我国8个燕麦种共21份材料的遗传差异及类群划分进行了研究。22个多态性引物在21个基因型间共扩增出114条带,其中97条带具有多态性,占85.1%。用这些条带计算了不同基因型间的遗传距离,发现各种间的遗传距离(0.053～0.510)普遍大于不同种内的遗传距离(0.022～0.087)。聚类结果显示,21份材料可划归为5大类群8个亚群,并与传统的分类结果基本一致。由此表明,用RAPD标记对燕麦进行遗传关系和分类研究是可行的。

The genetic diversity was detected using RAPD markers in 21 oat genotypes from 8 oat species, and the cluster analysis was undertaken. Using 22 polymorphic 10-mer arbitrary primers against 21 genotypes, a total of 114 fragments were amplified ,among which 97(85.1%) products were found to be polymorphic. These products were used to calculate genetic distance(GD),and it was indicated that the genetic distances between different species were generally larger than those within every species. The result of cluster analysis by using UPGMA method showed that the 21 genotypes may be classified into 5 groups which could be divided into 8 subgroups further, which was consistent with the classification result by using the traditional method. It was concluded that RAPD markers can be used on the studies of genetic relationships and classification of oat.