组织蛋白酶D在乳腺癌浸润转移中作用的研究

The Role of Cathepsin D on the Invasion and Metastases of Breast Cancer

目的 通过研究组织蛋白酶D与乳腺癌传统临床指标及部分生物学因子的关系 ,探讨组织蛋白酶D在乳腺癌浸润转移过程中的作用。方法 采用免疫组化SP法对组织切片进行染色处理。结果 16 0例乳腺癌组织Cath D的总表达率为 6 8.8% (110 / 16 0 ) ;Cath D表达与临床分期、肿块大小、组织学分级、c erbB 2、VEGF呈正相关 (P <0 .0 5 ) ,与ER、PR表达呈负相关 (均P <0 .0 2 5 ) ;与淋巴结转移、月经状况、年龄无相关性 (均P >0 .0 5 )。结论 Cath D过度表达与乳腺癌的浸润及转移有密切关系 ;Cath D促进肿瘤细胞转移的主要途径是血行播散而非淋巴道 ;ER、PR阴性患者中Cath D的高表达 ,提示应加强这部分病人的化疗及综合治疗。

Objective To investigate the role of cathepsin D in facilitating tumor cells invasion and metastases by comparing cathepsin D expression with clinical and biological indicators in breast cancer. Methods By immunohistochemical SP method. Results The positive rate of cathepsin D expression in all breast cancer patients was 68.8%(110/160). Cathepsin D expression was positive correlated with that of VEGF, c-erbB-2 as well as tumor size, clinical stage and histological grade (P< 0.01), but was negative correlated with the expression of ER and PR (P< 0.025). There was no relationships between cathepsin D and the following factors: metastasis of axillary lymph node, menopausal status and ages (P> 0.05). Conclusion The overexpression of cathepsin D is likely to be involved in the invasive and metastatic potential of breast cancer cells. The main pathway of cathepsin D promoting tumor cells metastasis is likely to be by blood circulation rather than by lympy road. The high overexpression of cathepsin D in ER or PR negative patients suggests that special adjuvant chemotherapy is needed to them.