三氧化二砷经肝动脉栓塞化疗对兔肝移植瘤的作用

Effects of Arsenic Trioxide by Transcatheter Arterial Chemoembolization on Rabbits with Hepatic Vx-2 Carcinoma

目的 探讨As2 O3 经肝动脉栓塞化疗对肝移植瘤的作用。方法 采用实验组 1(生理盐水 )、实验组 2 (碘化油 )、实验组 3(As2 O3 +碘化油 )经肝动脉注射治疗肝移植瘤一次 ,实验组 4 (As2 O3 )经肝动脉插管灌注药物 (连续 7天 )。观察肿瘤平均瘤重、平均瘤重抑制率、肿瘤细胞形态学变化 ,检测bcl 2 /bax基因表达和VEGF表达。结果 实验组平均瘤重分别为 16 .0 8± 2 .6 9g、3.4 4± 0 .84 g、1.5 2± 0 .2 6 g和4 .87± 0 .73g ,平均瘤重抑制率分别为 78.6 1%、90 .5 5 %和 6 9.71% ,实验组 3移植瘤瘤重低于其他实验组 (P <0 .0 5 )。实验组 3、4移植瘤组织bax基因表达显著上调、bcl 2基因表达显著下调 (P <0 .0 5 )、VEGF表达下调 (P <0 .0 5 ) ;电镜观察实验组 3、4均有典型的瘤细胞凋亡改变。结论 As2 O3 经肝动脉栓塞化疗对兔Vx 2肝移植瘤有显著抑瘤作用 ,碘化油能增强As2 O3 对肝移植瘤的抑制作用 ,两药具有协同作用。

Objective To evaluate the anticancer effect of arsenic trioxide(As 2O 3) by transcatheter arterial chemoembolization (TACE) on rabbits with hepatic Vx-2 carcinoma. Methods Forty New Zealand rabbits with hepatic Vx-2 carcinoma were managed with normal saline (experimental group 1), lipiodol and normal saline (experimental group 2), As 2O 3 and lipiodol (experimental group 3) by hepatic artery infusion respectively, whereas experimental group 4 was treated with As 2O 3 by TAI for 7 days consecutively. Four weeks later, the tumor weighty and its growth inhibitory rate was assessed respectively. The morphological changes of the tumor were observed under transmission electron microscope. bax/bcl-2 and VEGF expression were examined by immunohistochemistry. Results Average weighty of the tumor were 16.08± 2.69 g, 3.44± 0.84 g, 1.52± 0.26 g and 4.87± 0.73 g, and it's growth inhibitory rate in experimental group 2,3,4 were 78.61%, 90.55% and 69.71% respectively. The growth of tumor were obviously inhibited in experimental group 3 compared with the experimental group 1,2,4 (P< 0.05). The upregulated bax expression and downregulated bcl-2 expression were detected in experimental group3,4 by immunohistochemistry, comparison with experimental group 1,2 (all P< 0.05). Significantly decreased VEGF expression was also observed in experimental groups (P< 0.05). Some tumor cells of experimental group3,4 were showed the typically morphological characteristics of apoptosis under transmission electron microscope. Conclusion As 2O 3 by TACE can effectively inhibit hepatic Vx-2 carcinoma cells on rabbit's. Lipiodol strongly potentiates As 2O 3 inhibited the growth of hepatic Vx-2 carcinoma cells on rabbit,s, and the two drugs can produce a significant synergic effect.