奥曲肽对胃癌细胞株SGC7901细胞生长抑制作用及其机制的探讨

Effect and Mechanism of Octreotide on the Viability of Gastric Cancer Cell Line SGC7901 Cells

目的 研究生长抑素类似物奥曲肽对胃癌细胞株SGC790 1细胞的增殖、蛋白激酶B及端粒酶活性的影响。方法 应用奥曲肽作用于胃癌细胞株SGC790 1细胞不同时间后 ,检测其对体外培养胃癌细胞的抑制率 ,胃癌细胞蛋白激酶B(Akt/PKB)及端粒酶的活性。结果 奥曲肽对胃癌细胞增殖有明显抑制作用 ,呈剂量依赖性。同时能显著抑制胃癌细胞Akt/PKB的活性和端粒酶的活性 (12 ,2 4 ,4 8h均P <0 .0 1)。结论 生长抑素对胃癌细胞株SGC790 1细胞增殖有抑制作用 ,其机制与抑制Akt/PKB及端粒酶活性有关。

Objective To study the effect of octreotide on cell viability, protein kinase B and telomerase activity in human gastric cancer cell line SGC7901.Methods After incubation for 0,12,24,48 hours in different concentration of octreotide, MTT assay was used to determine the cell viability. Akt/PKB and telomerase activities were respectively detected. Results SGC7901 cells exhibited a dose-dependent inhibition of growth. The Akt/PKB and telomerase activity of SGC7901 cells was significantly inhibited by octreotide for 12, 24 and 48 h(P< 0.01). Conclusion The antiproliferative effect of octreotide on SGC7901 cells might be mediated by the inhibition of Akt/PKB and telomerase.