抑郁大鼠结肠组织P物质和血管活性肠肽的异常

Abnormalities of brain-gut peptides substance P and vasoactive intestinal peptide in colon of depressive rats

目的:观察抑郁症对大鼠结肠组织和血浆中脑肠肽类P物质和血管活性肠肽水平的影响。方法:按照Open-Field行为学评分方法,选取20只wistar大鼠,随机分成对照组和抑郁模型组(每组10只),利用长期不可预见的温和应激刺激建立大鼠抑郁模型。采用放射免疫方法分别检测大鼠血浆和结肠组织中P物质和血管活性肠肽的含量。结果:与正常组比较,模型组大鼠的垂直运动犤(13.00±4.21)次/min犦、水平运动犤(34.10±9.07)次/min犦和糖水消耗量犤(28.02±8.91)mL/24h犦均减少,差异有显著性意义(t=6.13,7.37,5.76,P均<0.01);血浆和结肠组织中P物质的含量犤分别为(73.82±15.19)和(50.31±12.09)ng/L犦显著增加,差异有显著性意义(t=-4.95,-7.45,P均<0.01);血管活性肠肽的含量犤分别为(6.39±0.76)和(1.37±0.42)ng/g犦显著降低,差异有显著性意义(t=2.80,4.91,P<0.05～0.01)。结论:抑郁症可能通过影响大鼠结肠组织中P物质和血管活性肠肽的释放而导致大鼠结肠的功能的异常。

AIM: To investigate the effects of depression on the contents of brain gut peptides substance P (SP) and vasoactive intestinal peptide (VIP) in plasma and colon of rats.<METHODS: Twenty Wistar rats, selected by Open Field behavioral scores, were randomly divided into control group(n=10) and depression model group(n=10).Rat models of depression were established by using the chronic mild stimulations. The contents of SP and VIP in plasma and colon of the rats were detected by radioimmunoassay respectively. <RESULTS: The rearing, crossing and sucrose consumption of the rats in the model group [(13.00± 4.21), (34.10± 9.07) times per minute, (28.02± 8.91) mL per 24 hours] were significantly decreased as compared with those in the control group (t=6.13, t=7.37, t=5.76, P< 0.01). The contents of SP in plasma and colon in the depression model group[(73.82± 15.19) and (50.31± 12.09) ng/L] were significantly increased (t=- 4.95, - 7.45, P< 0.01), while the VIP levels in plasma and colon [(6.39± 0.76) and (1.37± 0.42) ng/g] were significantly decreased as compared with those in the control group(t=2.80, 4.91,P< 0.05 to 0.01).<CONCLUSION: The changes of SP and VIP levels in plasma and colon may be related to the abnormality of colon presented in depressive rats.