灯盏花注射液和硝苯吡啶对急性心肌缺血大鼠心肌脂质过氧化的影响

Effect of breviscapine solution and nifedipine on lipid peroxidation of acute myocardial ischemic rats

目的:研究治疗心肌缺血损伤药物时,发现许多药物具有保护心肌的协同作用。研究灯盏花注射液、硝苯吡啶单用及并用对急性心肌缺血大鼠心肌脂质过氧化的影响,探讨其保护心肌的机制。方法:垂体后叶素致大鼠心肌缺血模型,观察灯盏花注射液及硝苯吡啶对大鼠心脏超氧化物歧化酶(superoxidedismutase,SOD)活力、丙二醛含量和一氧化氮含量的影响。用邻苯三酚自氧化法测定心脏SOD活力;用TBA法测定丙二醛含量,用改良的Griess法测定一氧化氮含量。结果:灯盏花组SOD活力为(383.91±201.21)nkat/L,心肌缺血组SOD活力(184.70±165.20)nkat/L,灯盏花注射液使心肌缺血大鼠心脏SOD活力明显增加(t=2.164,P<0.05)。灯盏花组一氧化氮含量(13.001±1.633)mg/g,心肌缺血组一氧化氮含量(15.625±2.560)mg/g,两组比较差异有显著意义(t=2.444,P<0.05),硝苯吡啶使心肌缺血大鼠心脏SOD活力显著增加硝苯吡啶组SOD活力(542.44±257.22)nkat/L,心肌缺血组SOD活力(184.70±165.20)nkat/L,(t=3.310,P<0.01)。结论:灯盏花注射液、硝苯吡啶对急性心肌缺血大鼠心肌脂质过氧化有一定影响。

AIM:When the drugs of the treatment for myocardial ischemic injury are studied, the coordinative effects on the therapy of myocardial ischemia in drugs are found.This study aims to investigate the effects of breviscapine solution and nifedipine on lipid peroxidation of acute myocardial ischemic rats,so as to study the mechanism of myocardial protection.<METHODS:The acute myocardial ischemic models were induced by pituitrin.The effects of breviscapine solution and nifedipine on the activity of superoxide dismutase(SOD) were observed, and the contents of malondialdehyde(MDA) and nitric oxide(NO) in hearts of rats were also observed.The activity of SOD was assayed with Trihy droxy benzene,the content of MDA was assayed with thin bar bituric scid(TBA),and the content of NO was assayed with Griess method.<RESULTS:The activity of SOD was(383.91± 201.21) nkat/L in breviscapine group and(184.70± 165.20) nkat/L in myocardial ischemic group.The activity of SOD in hearts of myocardial ischemic rats was improved markedly with breviscapine solution(t=2.164,P< 0.05).The content of NO was(13.001± 1.633) mg/g in breviscapine group and(15.625± 2.560) mg/g in myocardial ischemic group,which was significant difference between these two groups (t=2.444, P < 0.05).Nifedipine improved the activity of SOD in hearts of myocardial ischemic rats remarkably, that was (542.44± 257.22) nkat/L in nifedipine group and(184.70± 165.20) nkat/L in myocardial ischemic group(t=3.310, P< 0.01).<CONCLUSION:Breviscapine solution and nifedipine can influence lipid peroxidation of acute myocardial ischemic rats.