金匮肾气丸对糖尿病大鼠红细胞超氧化物歧化酶活性和血清丙二醛浓度的影响

Effects of jinkui shenqi wan on the activity of red blood cell superoxide dismutase and the concentration of serum malondialdehyde in diabetic rat

目的:观察金匮肾气丸对糖尿病大鼠红细胞超氧化物歧化酶(superoxidedismutase,SOD)活性和血清丙二醛浓度的影响。方法:将链尿佐菌素-糖尿病大鼠随机分成治疗组和糖尿病组,每组7只,以体质量、鼠龄相匹配的正常大鼠7只作为对照组。治疗组以金匮肾气丸22.5g/kg(2mL)灌胃,1次/d;糖尿病组和正常对照组灌胃自来水2mL/kg,1次/d,连续给药8周。观察各组大鼠SOD和丙二醛的变化。结果:糖尿病组SOD活性犤(5470.32±459.11)NU/L犦显著降低,丙二醛浓度犤(16.19±3.84)nmol/L犦显著升高,与正常对照组比较,差异均有显著性意义(P均<0.01)。治疗组SOD活性犤(7681.21±399.12)NU/L犦显著升高,丙二醛浓度犤(8.41±3.42)nmol/L犦显著下降,与糖尿病组比较,差异均有显著性意义(P<0.05～0.01)。结论:金匮肾气丸对糖尿病大鼠有抗过氧化损伤的作用。

AIM: To study the effect of jinkui shenqi wan(JKSQW) on the activity red blood cell superoxide dismutase(SOD) and the concentration of serum malondialdehyde(MDA) in diabetic rat.<METHODS: Streptozotocin induced diabetic rats were divided into two groups(treatment group and diabetic group) randomly, 7 rates for each group,and 7 normal rats matched in body weight and age were used as control group. The rats in treatment group were intragastrically administered with JKSQW(22.5 g/kg) one time a day. The rats in diabetic group and control group were intragastrically administered with tap water (2 mL/kg), one time a day and continuous medication for 8 weeks. The activity of SOD and the concentration of MDA in all groups were investigated.<RESULTS: The activity of SOD reduced[( 5 470.32± 459.11)NU/L]and the concentration of MDA increased[( 16.19± 3.84)nmol/L]significantly in diabetic group than those in normal control group. There was significant difference between the two groups (P< 0.01). The activity of SOD increased [( 7 681.21± 399.12)NU/L]and the concentration of MDA reduced[( 8.41± 3.42) nmol/L]in treatment group, There was significant difference as compared with diabetic group(P< 0.05- 0.01). <CONCLUSION: JKSQW has the effect of anti peroxidation in diabetic rat.