一种利用Taq酶快速标记DNA探针的方法

A Technique for Radiolabeling DNA using Taq DNA Polymerase

目的 :探索低成本、高效、快速的DNA探针标记方法。方法 :以特定引物和 16mer的随机引物作为延伸引物 ,利用Taq酶标记DNA探针。以大肠杆菌Klenow片断随机引物延伸标记法作为对照。点杂交方法检测探针标记效果。结果与结论 :Taq酶标记法和大肠杆菌Klenow片段随机引物延伸标记法同样有较好的标记效果 ,且随机引物或特定引物作为延伸引物均可以合成足够有效的探针。Taq酶标记法是一种低成本、高效。

Objective:The aim was to develop a low-cost,high-efficiency and rapid technique for DNA radiolabeling.Methods:Based on PCR principle,DNA was labeled using specific and random primers.The E.coli Klenow fragment random primer DNA labeling system served as control.Effect of labeling was detected by dot blot hybridization.Results and conclusion:Taq DNA Polymerase labeling method is as efficient as Klenow fragment random primer DNA labeling system.It is a low-cost,high-efficiency and rapid DNA radiolabeling method.