高空气球飞行对凤尾鸡冠花抗氧化功能的影响

Effects of High Altitude Balloon Flight on the Antioxidation of Water Extract from Celosia cristata L.cv.Plumosa

目的 :探讨高空气球飞行实验对鸡冠花抗氧化功能的影响。方法 :利用高空气球搭载了凤尾鸡冠花 (CelosiacristataL .cv.Plumosa)种子 ,进行空间诱变处理。飞行高度 4 0 .112km ,飞行时间近 4h ,回收后播种栽培 ,采收子一代 (SP1)花序 ,叶片。采用比色法研究搭载组和对照组花序、叶片水提取液对羟自由基 (·OH)和超氧阴离子自由基 (O·2 )的清除作用。同时 ,分别测定分析了各组样品VC、黄酮醇的含量和SOD活力 ,据此探索在高空环境影响后样品抗氧化活性变化与自由基清除剂含量之间的关系。结果 :各组水提取液均能降低·OH引发的还原型细胞色素C[Cyt·C(Ⅱ ) ]氧化作用和O·2 对羟胺的氧化作用 ,而两种搭载组样品水提取液清除活性氧的能力均比对照组显著提高。结论

Objective:To investigate the effect of high altitude balloon flight test on the antioxidation of water extracts from Celosia cristata L.Methods:The seeds of Celosia cristata L.cv.Plumosa were carried by a high-altitude balloon(CHAB) for space mutation,flying to an altitude of 40.112km from sea level for about 4 hours.After returning to the ground,the treated seeds were cultivated on the ground and the inflorescences and leaves of SP1 generation were collected and used for water extraction.The effects of water extracts from inflorescences and leaves of CHAB groups and the control groups on scavenging hydroxyl radical(·OH) and superoxide radical(O_·2(^-)) were studies by colorimetric method,respectively.The contents of VC,flavonols and activity of SOD of all samples were also analyzed and determined,respectively.Then,the relation between the antioxidation activity of samples and the contents and activity of free radical scavengers in samples was investigated after the effect of high altitude environment.Results:The water extracts of all samples could reduce ·OH initiated oxidation of reductive Cyt·c(Ⅱ) and inhibit O_·2(^-) initiated oxidation of hydroxylamine.But,the abilities of scavenging active oxygen of 2 kinds of water extracts from CHAB groups were significantly higher than those of the control.Conclusion:The evident effects of high space environment condition mutation on the synthesis of the antioxidation active substances and antioxidation capability in C.cristata L.were observed.