慢性牙龈炎及慢性牙周炎治疗前后患牙龈沟液中t-PA及PAI活性的比较

Comparion the activity of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor (PAI) in gingival crevicular fluid before and after treatment from chronic gingivitis and chronic periodontitis

目的 :比较慢性牙龈炎、慢性牙周炎患牙治疗前后龈沟液中t PA及PAI活性。方法 :选择慢性龈炎(n =2 8)及慢性牙周炎 (n =5 2 )患牙两组 ,在治疗前及治疗后收集龈沟液 (GCF)并记录临床指标 ,用发色底物法测定GCF中t PA及PAI的活性。结果 :t PA活性在牙龈炎组治疗前后差异无显著性 ,在牙周炎组治疗前后差异有显著性 ;PAI活性在牙龈炎组及牙周炎组治疗前后差异均有显著性 ;PAI/t PA比值在牙周炎组治疗前后差异有显著性 ,牙龈炎组治疗前后差异无显著性。结论 :PAI可以作为判断牙龈炎症程度及治疗效果较为客观的指标 ;牙周炎组中治疗后PAI/t PA显著降低的部位是否预示较差的预后 ,尚需进一步纵向研究。

Objective:To investigate the activity of t-PA and PAI before and after initial periodontal therapy in gingival crevicular fluid from chronic gingivitis and chronic periodontitis.Method: Seven gingivitis and thirteen periodontitis patients were selected and experienced initial periodontal therapy. Gingival crevicular fluid from 80 sites were collected before and after treatment, which includes 28 sites from seven gingivitis, 52 sites from thirteen periodontitis. Result: The results showed that there was no significant difference of the activity of t-PA before and after treatment in gingivitis but there was significant differeoce before and after treatment in periodontitis sites; there was significant difference in PAI level before and after treatment both in gingivitis and periodontitis. The ratio of PAI/t-PA was significant differences before and after treatment in periodontitis, but not in gingivitis.Conclusion: This study indicated the activity of PAI may be objective indicator to judge the inflammation of periodontium and treatment effect. The decrease of the ratio of PAI /t-PA in periodontitis warrants further investigation via longitudinal studies to assess whether it can be used as an indicator of the poor prognosis.