白藜芦醇对离体豚鼠乳头状肌的电生理效应(英文)

Electrophysiological effects of resveratrol on guinea pig papillary muscles

本文旨在应用标准玻璃微电极技术,观察白藜芦醇对离体豚鼠乳头状肌的电生理效应。结果显示:(1)白藜芦醇(30、60、120 μmol/L)可剂量依赖性地缩短乳头状肌细胞的动作电位时程;(2)对部分去极化的乳头状肌,白藜芦醇(60 μmol/L)不仅缩短动作电位时程, 而且降低动作电位的幅值和超射值, 减慢零期最大上升速度;(3)用无钙K-H 液灌流标本可完全取消白藜芦醇对乳头状肌细胞的作用;(4)钾通道开放剂四乙基氯化铵(TEA, 20 mmol/L), 不能阻断白藜芦醇的电生理效应;(5)预先应用一氧化氮合酶抑制剂L-NAME(1 mmol/L), 对白藜芦醇的上述效应无影响。以上结果表明,白藜芦醇可缩短正常乳头状肌细胞动作电位时程,这一效应可能与其抑制钙离子内流有关,但此作用机制中 NO 的作用并不显著。

The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded by using intracellular microelectrode technique. The results obtained are as follows: (1) In normal papillary muscles, resveratrol (30, 60, and 120 μmol/L) shortened the duration of action potential (APD) in a concentration-dependent manner. (2) In partially depolarized papillary muscles, resveratrol ( 60 μmol/L ) not only shortened APD, but also decreased the amplitude of action potential ( APA), overshoot ( OS ) and maximal rate of depolarization in phase 0 ( Vmax ). (3) Perfusion with Ca2+-free K-H solution, completely abolished the effects of resveratrol (60 μmol/L) on papillary muscles. (4) Applica- tion of potassium channel blocker tetraethylammonium chloride (TEA, 20 mmol/L) did not prevent the effect of resveratrol (60 μmol/L) on action potential. (5) Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the effect of resveratrol (60 μmol/L). All these results indicate that the electrophysiological effects of resveratrol on guinea pig papillary muscles are likely due to the reduction of calcium influx, which might not be mediated by NO.