残胃粘膜幽门螺旋杆菌感染与COX-2 mRNA表达的关系

The Relationship between Helicobacter Pylori Infection and Expressions of Cyclooxygenase-2 mRNA in the Remnant Stomach Mucosa

目的:探讨不同部位残胃粘膜幽门螺旋杆菌(Helicobacterpylori,Hp)感染与环氧化酶-2(COX-2)mRNA表达的关系。方法:残胃患者均行胃镜检查,于吻合口和胃体大弯处采用两点取材的方法进行胃镜活检,活检标本分别进行Hp感染检测和通过定量PCR(Real-timeSemi-quantitativePCR)方法检测COX-2mRNA表达。结果:本研究收集残胃患者共58例,Hp感染阳性者为38例,占65.5%。残胃吻合方式为BillrothI(BI)式吻合者34例,残胃吻合口处和残胃体部Hp感染阳性COX-2mRNA表达均明显高于Hp感染阴性的表达(P<0.05);残胃吻合方式为Bill鄄rothII(BII)式吻合者24例,无论残胃体部还是残胃吻合口处Hp感染阳性COX-2mRNA表达也明显高于Hp感染阴性的表达(P<0.05);Hp感染阴性者20例,BI式吻合和BII式吻合各10例,包括残胃吻合口处和残胃体部,BI式吻合COX-2mRNA表达明显高于BII式吻合的表达(P<0.05)。结论:残胃粘膜COX-2mRNA表达与Hp感染有关,吻合方式也影响COX-2mRNA的表达。

Objective: To explore the relationship between helicobacter pylori (Hp)infection and expressions of cyclooxygenase-2(COX-2) in the remnant stomach mucosa. Methods: The patients with gastrectomy were examined by upper gastrointestinal endoscopy. The biopsy specimens were obtained from stoma and the greater curvature of the upper corpus to assess for Hp (by H.E. and Giemsa staining) and conduct real-time semi-quantitative PCR. mRNA was extracted from biopsy specimens to measure the COX-2 gene mRNA levels by real-time PCR method. Results: Our results revealed that Hp infection rate in the remnant stomach mucosa were 65.5%(38/58). In 34 patients with BI anastomosis, COX-2 levels in stoma and corpus were significantly higher in Hp-positive patients than in Hp-negative patients;In 24 patients with BII anastomosis, COX-2 levels in Hp-positive patients were significantly higher than those in Hp-negative patients, including in stoma and in corpus. In 20 Hp-negative patients, COX-2 levels in BI anastomosis were significantly higher than those in BII anastomosis. Conclusions: Hp infection induces COX-2 mRNA expressions in the remnant stomach mucosa; The types of anastomosis also influence COX-2 mRNA expressions.