缺磷对长豇豆幼苗ZRs含量的影响

Effects of Phosphorus-deficiency Stress on ZRs Contents in Asparagus Bean Seedlings

为了研究耐缺磷程度不同的 3种长豇豆幼苗在缺磷胁迫下ZRs含量的变化 ,选用耐缺磷程度不同的长豇豆品种 :芦花白、香港青和二芦白的幼苗作为材料 ,设供磷和缺磷 2个处理 ,取供试幼苗的根系、最嫩完全展开叶、老茎叶、嫩茎叶作样品 ,采用ELISA方法进行测定。结果表明 ,缺磷胁迫下长豇豆幼苗各部位ZRs含量都下降 ,根系和老茎叶ZRs含量降幅二芦白 >芦花白 >香港青 ;最嫩完全展开叶降幅为 :芦花白 >香港青 >二芦白 ;嫩茎叶降幅为 :芦花白 >二芦白 >香港青。指出缺磷胁迫下长豇豆幼苗生长受抑制可能与ZRs含量有关 ,其中二芦白老茎叶和根系衰老较严重 ,生长受抑制也较严重。

Changes of zeatin ribosides (ZRs) contents in seedlings of 3 asparagus bean (Vigna unquiculata W.ssp.sesquipedalis (L.) Verd) cultivars differing in tolerance to phosphorus-deficiency were studied under phosphorus-deficiency stress.There were 2 treatments:+P(1 m mol.L -1P) and -P(0 mmol.L -1P).The ZRs contents in root,youngest fully-expanded leaf,older leaf and stem and younger leaf and stem of asparagus bean seedling were measured by ELISA.The results showed that ZRs contents in asparagus bean seedling decreased under P-deficiency stress.The decrease of ZRs contents in older leaf and stem and root of cv.Erlubai(Low P-deficiency-tolerance)>cv.Luhuabai (High P-deficiency-tolerance)>cv.Xianggangqing (middle P-deficiency-tolerance).The decrease of ZRs content in youngest fully-expanded leaf of cv.Luhuabai>cv.Xianggangqing>cv.Erlubai.The decrease of ZRs contents in younger leaf and stem of cv.Luhuabai>cv.Erlubai>cv.Xianggangqing.There was relationship between seedling growth suppression and decrease of ZRs contents,thus senescence of root and older stem and leaf was more serious in cv.Erlubai and the growth were suppressed more in cv.Erlubai.