木质素酚吸附内毒素的研究

Research on the Adsorption of Endotoxin by Lignophenol

内毒素是革兰氏阴性菌细胞壁的外层脂多糖组分,可引起机体发热反应,重症将导致休克.内毒素对热或有机溶剂都十分稳定,通常的灭菌操作难以使之除去.研究结果表明,采用室温相分离法从木材中分离制得的木质素-酚的衍生物能吸附内毒素.在室温下,50 mg木素酚可以吸附存在于水溶液中的内毒素1000～2000 EU.加入有机溶剂后木素酚释放出被它吸附的内毒素.分离纯化后的木素酚又可以重新吸附内毒素.木素酚有望作为吸附、清除内毒素的功能性材料在医疗行业推广应用.

Endotoxin is composed of lipopolysaccharid, which is the outer cell-wall component of Gram-negative bacterials. Endotoxin leads organism to fever, in serious case it leads up the patient to shock. Endotoxin is very stable to heat or organic solvents, the usual sterile operation is difficult to remove endotoxin. The result of the research shows that lignophenol, which was separated from milled wood in phase-separate reaction system composed of phenol derivative and concentrated acid, can adsorb endotoxin. At room temperature, 50 mg lignophenol can adsorb 1000~2000 EU endotoxin in aqueous solution. The adsorbed endotoxin was released when organic solvent was added to the system. Separated lignophenol from lignophenol-endotoxin complex can adsorb endotoxin again. Lignophenol is a hopeful functional material for removing endotoxin in medical industry.