纤维素酶水解纤维素类废弃物的研究

Study on Hydrolysis of Cellulose Waste by Cellulase

研究了纤维素酶水解纤维废弃物的适宜条件。研究结果表明,在底物浓度80 g/L,pH 4.8,酶解温度50℃,酶用量100 IU/g(对底物),酶解时间60 h条件下,处理纤维废弃物,可以得到较高的还原糖浓度,酶解液还原糖浓度可达到16.44 mg/mL,酶解液及残渣可分别进一步加以利用。

The optimum conditions of hydrolysis of cellulose waste by cellulase were studied. The results showed that the optimum conditions were substrate consistency 80 g/L, pH 4.8, cellulase hydrolysis temperature 50℃, cellulase usage 100 IU/g(substrate), cellulase hydrolysis time 60 h. Under the optimum conditions, reducing sugar consistency could be best to 16.44 mg/mL. The hydrolysate and the residual solids will be used.