环戊通松弛豚鼠睫状肌的调节作用

A study of the effect of cyclopentolate cycloplegic on guinea pigs

目的 :确定在对豚鼠进行屈光检查时应用环戊通所需的点药次数及其对睫状肌麻痹的作用时间。方法 :本实验共 5 8只豚鼠 ,分三组 :第一组比较豚鼠点药前后屈光状态的变化 ,第二组比较点药 2次和 4次时 30min和 6 0min时的调节变化 ,第三组研究点药四次后环戊通对睫状肌麻痹作用随时间的变化趋势 ,逐步确定对豚鼠检影时是否需要点药、点药次数及点药后的最佳检影时间。结果 :点药和未点药时豚鼠的平均屈光度比较显示 ,点药后屈光度偏向正 ,有统计学意义 (P <0 .0 5 ) ;且调节变化在点药后稳定 ,差异有显著性(P <0 .0 5 ) ;点 2次药和点 4次药后 30min时的调节变化比收稿日期 :2 0 0 4-0 8-0 2 ;修回日期 :2 0 0 4-10 -15基金项目 :国家自然科学基金项目 (3 0 3 715 0 7) ;浙江省自然科学基金项目 (3 0 2 0 95 ,ZB0 2 0 2 ) ;浙江省卫生厅重大课题项目 (2 0 0 2ZD0 0 9) ;浙江省科技厅科技攻关计划 重点科研项目 社会发展项目(2 0 0 3C2 3 0 0 5 )。作者简介 :蒋丽琴 (1973 -)女 ,山西太原人 ,在读硕士研究生 ,研究方向 :视觉光学。E -mail:jianglq_1@hotmail.com6 0min时大 ,且点药 2次比点药 4次的调节变化大 ,差异有显著性 (P <0 .0 5 ) ;在点完第 4次药之后 6 0到 90min时检影效果最好 ,并得出其相关?

Objective:To determine the efficacy and time course of cyclopentolate cycloplegia in neonatal guinea pigs used in experimental studies of refractive development.Methods:Three groups of three week old guinea pigs were used as subjects. Refractive states were monitored with streak retinoscopy. The subjects were grouped as following:①Group 1,the refractive states before and after cycloplegia were compared.②Group 2,accommodative changes were compared using cycloplegic doses of 2 drops(5 min interval) with that of 4 drops.③Group 3,accommodative changes were recorded at 30 min,60 min,90 min and 120 min after drops of cyclopentolate were topically applied 4 times at 5 minute intervals.Results:The accommodative state was stabilized after topical application of 1% cyclopentolate. Four drops at 5 min. intervals worked well. A wait of 60 to 90 minutes after cyclopia is recommended for a guinea pig model of myopia.Conclusion:Cycloplegia is necessary and efficient for examining the refractive state of neonatal guinea pigs. Optimal results for refraction can be obtained in 60 to 90 minutes after 1% cyclopentolate is topically applied in 4 drops at intervals of 5 minutes. [