红丝线草提取物对高血压合并高脂血症模型大鼠降压作用的研究

Effects of Peritrophe roxburghiana on Blood Pressure in Renal Hypertensive and Hyperlipidemic Rats

目的 :观察红丝线草提取物对肾性高血压合并高脂血症模型大鼠 (renalhypertensiveandhyperlipidemicrat,RHHR)血压和血清一氧化氮 (nitricoxide ,NO)、血浆及胸主动脉组织中血管紧张素Ⅱ (angiotensinⅡ ,AngⅡ )含量的影响。方法 :采用双肾双夹结合高脂乳剂灌胃法复制RHHR模型。大鼠造模后 ,连续 5周灌胃给予红丝线草提取物 ,给药前及给药后每周末测定血压 ,并于第 5周末测定血压后处死大鼠 ,取血测NO及AngⅡ ,另取胸主动脉测组织AngⅡ的含量。结果 :红丝线草提取物灌胃 1～ 2周后 ,血压开始明显下降至连续给药 5周末 ;降压的同时 ,胸主动脉组织中的AngⅡ明显下降 ,而血浆中AngⅡ的含量无明显变化 ;同时测得血清NO水平明显升高。结论 :红丝线草提取物能明显降低RHHR的血压 ,其降压机制可能与升高血清NO浓度和降低血管组织中的AngⅡ含量有关。

Objective:To observe the effects of Peristrophe roxburghiana (HSX) on the blood pressure in renal hypertensive and hyperlipidemic rats (RHHR),and investigate the possible mechanisms of its pharmacological effects Methods:The two-kidney,two-clip method was used to produce the renovascular hypertensive rats,then high lipid emulsion was administered to produce the model of hyperlipidemic rats Drugs were administered for 5 weeks Blood pressure was measured weekly before and after administration After rats were killed at the end of the 5 th week,blood was sampled to measure plasma angiotensin Ⅱ and serum NO. Angiotensin Ⅱ in the thoratic aorta was measured too Results:The BP decreased significantly to the end of 5 th week (P<0 05) after treatment of 1～2 weeks with HSX HSX(H or L dosage) increased the level of serum NO evidently at the end of the experiment No significant statistical difference was found in the level of plasma angiotensin,but HSX(H) and captopril decreased the value of angiotensin Ⅱ in blood vessel (P<0 01) Conclusion:HSX decreased the blood pressure significantly for RHHR and the mechanism of its antihypertensive effect was possibly related to the increasing of serum NO and the decreasing of angiotensin Ⅱ in the thoratic aorta