红毛五加多糖的基本性质研究

STUDY ON CHARACTERIZATION OF POLYSACCHARIDES FROM ACANTHOPANAX GIRALDII HAMS

本文从红毛五加中分离得到了三种多糖 ,并对它们的基本性质进行了研究。红毛五加经热水浸提 ,用Sevag法脱蛋白 ,经DEAE cellulose 32离子交换层析和SephacrylS 2 0 0HR凝胶层析纯化得到三个主要多糖组分 :AHP I、AHP II和AHP III。HPLC测定AHP I、AHP II和AHP III的表观分子质量分别为 7.0kD、5 9.5kD和 12 .9kD。红外光谱分析三种多糖具有多糖类物质的一般特征。经纸层析和GC结果综合分析得知 ,AHP I含有阿拉伯糖、岩藻糖、甘露糖、葡萄糖、半乳糖 ,其摩尔比为 1.0 0∶1.4 0∶0 .6 4∶3.13∶2 .0 9;AHP II含有鼠李糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖 ,其摩尔比为 0 .36∶1.0 0∶0 .15∶0 .2 0∶1.10 ;AHP III含有鼠李糖、阿拉伯糖、岩藻糖、甘露糖、葡萄糖和半乳糖 ,其摩尔比为 1.2 2∶1.0 0∶0 .4 0∶0 .2 8∶0 .2 6∶1.0 3。咔唑 硫酸法测得AHP II、AHP III的糖醛酸含量分别为 6 .7%和 6 .0 % ;AHP I不含糖醛酸。体内试验表明 ,红毛五加粗多糖对小鼠S180 肉瘤有明显抑制作用。

Three water-solube polysaccharides,AHP-I,AHP-II and AHP-III from the cortices of Acanthopanax giraldii Hams,were isolated and purified by ion-exchange chromatographies on DEAE-cellulose 32 and Sephacryl S-200.Three fractions were shown to be homogeneous by cellulose acetate membrane electrophoresis,agarose electrophoresis and HPLC.The apparent average molecular weight of AHP-I,AHP-II and AHP-III was estimated to be approximately 7.0 kD,59.5 kD and 12.9 kD respectively.Normal characterizations were found from IR.The components of three kinds of polysaccharides were identified by gas chromatography:rhamnose,arabinose,mannose,glucose and galactose were found to be in the molar radio of 0.36∶1.00∶0.15∶0.20∶1.10 in AHP-II;rhamnose,arabinose,fucose,mannose,glucose and galactose were found to be in the molar radio of 1.22∶1.00∶0.40∶0.28∶0.26∶1.03 in AHP-III and arabinose,fucose,mannose,glucose and galactose were found to be in the molar radio of 1.00∶1.40∶0.64∶3.13∶ 2.09 in AHP-I.The content of uronic acid in AHP-II,AHP-III was 6.7% and 6.0% respectively,and there was no uronic acid in AHP-I.The experimentatal results indicated that the crude polysaccharide from Acanthopanax giraldii Hams ip 200 mg/kg inhibited the sarcoma proliferation of Sarcinoma 180.