摘要
研究了碳源和氮源对白腐菌Sarcodonasparatus合成脂肪酶的影响。橄榄油是脂肪酶合成的最佳碳源,脂肪酶活力达0.65U/mL。蛋白胨、尿素和氯化铵对脂肪酶的合成有促进作用,它们的酶活力分别为0.65、0.67和0.62U/mL,而硫酸铵抑制脂肪酶的合成,其酶活力仅为0.08U/mL。C/N比对细胞生长和酶的合成影响很大,当C/N比从2增加到10时,脂肪酶活力从0.65下降到0.14U/mL,而细胞浓度从3.08升高到4.58mg/mL。在培养基初始pH值5.5、28℃下培养,S.asparatus合成的脂肪酶活力最高,脂肪酶活力和细胞浓度分别为0.65U/mL和3.08mg/mL。
Carbon sources and nitrogen sources affecting lipase production by Sarcodon asparatus were investigated.Lipase activity was the highest(0.65 U/mL) for olive oil compared with other carbon sources.Lipase production was stimulated by peptone(0.65 U/mL),urea(0.67 U/mL) and ammonium chloride(0.62 U/mL),and repressed by (ammonium) sulfate with a low lipase activity of 0.08 U/mL.C/N ratio was a significant factor for lipase production and cell growth.When C/N ratio was raised from 2 to 10,lipase activity decreased from 0.65 to 0.14 U/mL,and biomass increased from 3.08 to 4.58 mg/mL.The optimal culture conditions of S.asparatus were pH value 5.5 and 28 ℃,and lipase activity was 0.65 U/mL with a biomass concentration of 3.08 mg/mL.
出处
《林产化学与工业》
EI
CAS
CSCD
2004年第4期1-6,共6页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目 (3 0 1 0 0 1 42 )
