摘要
对经氨预处理的大豆秸秆纤维素固态发酵产酶及酶解产糖工艺过程进行了研究,影响康氏木霉(Trichodermakoningii)固态发酵的主要因素是温度、pH值、培养基液固质量比及培养时间,影响纤维素酶水解的条件主要是温度、pH值及时间,结果表明:较适宜的产酶条件为温度30℃,pH值5.0,培养基液固质量比2.5∶1,时间96h,产纤维素酶活力为798.84FPU/mL,以所产纤维素酶进行酶水解,较适宜条件为温度55℃、pH值5.6、时间36h,酶解率为18.98%。由液相色谱分析可知酶解液的主要成分为葡萄糖、纤维二糖和木糖,为大豆秸秆的进一步综合利用提供了参考数据。
Solid-substrate fermentation and enzymolysis of soybean stalk cellulose pretreated with ammonium had been studied.The major affecting factors of solid-substrate fermentation with the cellulase produced by Trichoderma koningii are temperature,pH value,medium moisture content and time.The major factors affecting enzymolysis are pH value,temperature and time.It showed that the adequate condition for enzyme production is temperature 30 ℃,pH value (5.0),liquid to solid ratio 2.5∶1,time 96 h,and cellulase activity produced is 798.84 FPU/g.Using this cellulase for enzymolysis,the optimum condition is:temperature 55 ℃,pH value 5.6,36 h,the yield of enzymolysis is 18.98%.The composition of enzymolysis product was analyzed by liquid chromatography,which was composed of glucose,(xylose) and cellobiose.This study provides reference data for further comprehensive use of soybean stalk.
出处
《林产化学与工业》
EI
CAS
CSCD
2004年第4期107-110,共4页
Chemistry and Industry of Forest Products
基金
国家自然科学基金
中国节能投资公司联合研究基金资助项目(50278024)
关键词
大豆秸秆
康氏木霉
酶水解
固态发酵
soybean stalk
Trichoderma koningii
enzymolysis
solid-substrate
fermentation
