高效液相色谱法同时测定小儿感冒冲剂中对乙酰氨基酚和咖啡因的含量

Simultaneous Determination of Acetaminophen and Caffeine in Children's Suxiao Cold Granules by HPLC

目的:建立同时测定小儿速效感冒冲剂中对乙酰氨基酚和咖啡因含量的高效液相色谱方法.方法:采用HypersilODS C18柱(5μm,250 mm×4.6 mm),流动相为甲醇∶水(30∶70),检测波长为280 nm,峰面积外标法.结果:对乙酰氨基酚在10.08～100.80μg·ml-1,咖啡因在0.592～5.920μg·ml-1浓度范围内线性关系良好;对乙酰氨基酚回收率为98.9%,RSD为0.58%(n=6),咖啡因回收率为100.15%,RSD为2.52%(n=6).结论:该方法具有操作简便、结果准确等优点.

Objective: To establish a HPLC method for simultaneous determination of both acetaminophen and caffine in rhildren s Suxiao Cold granules. Method: Hypersil ODS C18 column (5μm,250 mm×4.0 mm)was used, the mobile phase consisted of metha-nol-water (30:70) , detecting wavelength was at 280 nm. Result: There were good linear correlation within a range of 10.08-100.80 μg·ml-1 of acetaminophen and 0.592-5.920 μg·ml-1 of caffeine. The average recoveries of acetaminophen, and caffeine were 98. 9%(RSD=0. 58% ) and 100. 2% (RSD = 1. 52% ) Respectively. Conclusion: The method is simple,accurate.