蛇床子素对植物病原真菌抑制机制的初步研究

Inhibition Mechanism of Osthol to Plant Fungus Pathogens

天然化合物蛇床子素(osthol)对草坪纹枯病菌Rhizoctoniasolani、芒果炭疽病菌Colletotrichummusae、苹果叶枯病菌Rhizoctoniasolani、辣椒疫霉病菌Phytophoracapsici、辣椒炭疽(红)病菌Collectotrumgloesporioides、番茄灰霉病菌BotrytiscinereaPers、油菜菌核病菌Sclerotiniasclerotiorum、小麦赤霉病菌Fusariumgraminearum、苹果轮纹病菌Macrophomakawatsukai、水稻稻瘟病菌Pyriculariagrisea、西瓜枯萎病菌Fusariumoxysporumf.sp.niveum、棉花枯萎病菌Fusariumoxysporumf.sp.vasinfectum、棉花黄萎病菌Verticilliumdahliae等有不同的抑制活性,对大多数病菌其抑制中浓度(EC50值)在21.15～61.62μg/mL之间。以小麦赤霉病菌为研究对象,结果表明50μg/mL蛇床子素对其孢子萌发有显著的抑制作用;100μg/mL蛇床子素处理24h后,菌丝大量断裂,可溶性蛋白含量增加,菌体葡萄糖含量呈"V"型变化,几丁质水解酶活性和几丁糖含量均高于对照。

Natural compound osthol has been proved to have a wide spectrum of fungi inhibition including Rhizoctonia solani, Colletotrichum musae, Rhizoctonia solani, Phytophora capsici, (Collectotrum) (gloesporioides,) Botrytis cinerea Pers, Sclerotinia sclerotiorum, Fusarium (graminearum,) Macrophoma kawatsukai, with the value of EC_(50) ranged from 21.15 μg/mL to (61.62 μg/mL) to some fungi. F. graminearuym was used to research the inhibition mechanism of osthol, the (results) showed 50 μg/mL osthol could inhibit the germination of its spores evidently. After treatment of (osthol) for 24 h, the hyphae of this fungus growing in aliqousculture came into large mounts of fragments. Glucose content decreased firstly and then increased to the level of control. Soluble proteins, (chitin) content and (chitinase) activity were higher than that of control.