小鼠直肠淋巴引流途径及淋巴结转移模型的初步探讨

The Study of the Mouse Rectal Lymphatic Drainage Routes and Lymph Node Metastasis Animal Model(A preliminary study)

目的 :初步探讨小鼠直肠淋巴引流途径及直肠肿瘤淋巴道转移规律。方法 :实验分为 2组 :第 1组 6只 C5 7BL / 6 J鼠通过直肠黏膜下注射 1× 10 6 鼠 B16黑色素瘤细胞观察肿瘤生长和转移情况。第 2组 30只昆明种小鼠通过直肠黏膜下注射 0 .0 5 m L美兰观察不同时间 (10、2 0、30、4 0、6 0 min)直肠淋巴引流情况。结果 :在第 1组中 ,所有实验动物都出现局部肿瘤生长 ,3周左右小鼠肿瘤平均直径 2 .2 cm (2 .0～ 2 .6 cm )开始死亡 ,5 / 6只小鼠出现不同程度的淋巴结转移 ,分别为髂动脉间 5 / 6、左髂动脉旁3/ 6、右髂动脉旁 3/ 6、左肾门 2 / 6、左腹股沟 3/ 6、右腹股沟 4 / 6。第 2组中 ,2 7/ 30只小鼠淋巴结出现不同程度的染色 ,染色情况如下 :髂动脉间 2 5 / 30、左髂动脉旁 2 1/ 30、右髂动脉旁 14 / 30、左肾门 5 / 30、右肾门 6 / 30 ,没有出现腹股沟染色淋巴结。结论 :两组实验小鼠直肠淋巴在腹腔内引流和转移途径基本一致 ,髂动脉间淋巴结可能为直肠引流第一站 (一级淋巴结 )、髂动脉旁淋巴结为第二站 (二级淋巴结 )、肾门淋巴结为第三站 (三级淋巴结 )。同时初步建立的小鼠直肠肿瘤淋巴道转移模型为其他相关研究提供了一个有用的工具 ,值得进一步探讨。

Objective:To discover the mouse rectal lymphatic drainage routes and the rules of the mouse rectal tumor metastasis via lymphatic.Methods:In the first group,female C57BL/6J mice(n=6) were inoculated in rectum with 1×10 6 B16 melanoma cells submucosally and observed the tumor growth and lymph nodes metastasis. In the second group,female KM mice (n=30) were injected in submucosal layer of the rectum with the methylene blue(0.05 ml) and observed the dyed lymph nodes in different times(10,20,30,40,60 min).Results:In the first group, all experimental animals developed local tumors. Approximately 3 weeks after tumor inoculation, the mice began to die and tumors average diameter was 2.2(range 2.0-2.6) cm and lymph nodes metastases occurred in 5/6 animals, interarterioiliac nodes 5/6,1eft iliac nodes 3/6,right iliac nodes 3/6,1eft para-renal nodes 3/6,right para-renal nodes 3/6,1eft inguinal nodes 3/6,right inguinal nodes 4/6, respectively. In the second group,27 of 30 mice lymph nodes were dyed, interarterioiliac nodes 25/30,1eft iliac nodes 21/30,right iliac nodes 14/30,1eft para-renal nodes 5/30,right para-renal nodes 6/30,respectively;no inguinal nodes were dyed.Conclusion:In the mouse rectal lymphatic drainage and neoplasm metastasis routes, interarterioiliac nodes may be the first level nodes; iliac nodes the second; para-renal nodes the third.At the same time,the animal model of rectal lymphatic metastasis provides a useful tool for other oncological researchs and is worthy of further study.