功能性β-二酮型高分子纳米微球的制备及其共振散射光性质

Preparation and Resonance Light Scattering Property of Functional β-Diketone Polymeric Nanoparticles

在超声辐照下 ,用过氧化氢对聚乙烯醇 (PVA)氧化降解 ,制得纳米尺寸的 β 二酮型高分子微球(PVK) ,红外光谱表征其 β 二酮型结构为烯醇式 ,透射电镜观察到该纳米粒子平均直径为 2 0nm。对PVK的共振散射光性质研究发现 ,pH =3 0时 ,PVK与蛋白质形成缔合微粒时将导致PVK 380nm处RLS信号急剧增加 ,其强度增加值与蛋白质的浓度呈线性关系 ,据此可以建立一种测定蛋白质的新方法。测得HSA和γ G的线性范围分别为 0～ 15 0× 10 -6g/mL和 0 2× 10 -6～ 8 5× 10 -6g/mL ,检出限为 1 4× 10 -9g/mL和 17 5× 10 -9g/mL。本法应用于人血清样品中蛋白质的定量分析 。

A kind of functional β-diketone polymeric nanoparticles(PVK) was prepared by an oxidation- degradation of PVK under ultrasonic radiation. The enol structures of PVK were characterized by IR spectroscopy. The nanoparticles are spherical with an average diameter of 20 nm. Studies on the resonance light scattering(RLS) properties of PVK show that the interaction of PVK with the proteins results in a great enhancement of RLS signals at 380 nm when pH=3.0. And the enhanced intensity of PLS at this wavelength is proportional to the concentration of the proteins. A protein determination method is thus proposed based on this property. Under optimal conditions, the linear ranges are o～15.0×10 -6 g/mL and 0.2×10 -6～8.5×10 -6 g/mL for HSA and γ-G, respectively. The detection limits are 1.4×10 -9 g/mL and 17.5×10 -9 g/mL for HSA and γ-G, respectively. The method has been applied to the analysis of total proteins in human serum samples with satisfactory result.