基于光谱自编码微球的多样品免疫荧光混合筛选和荧光成像

Immunofluorescence Mix-screening of Multi-samples and Fluorescence Imaging Based on Optical Self-encoding Microbeads

研究了微球粒度、温度、pH和包被液浓度对聚苯乙烯微球免疫吸附的影响。将20种编码微球与20个样品对映包被,进行免疫荧光混合筛选,分析荧光信号最强微球的光谱编码,确定了阳性样品的编号。其异硫氰酸荧光素(FITC)荧光标记抗体筛选灵敏度可达体积稀释比1∶2000。采用氩离子激光扫描荧光成像技术,成功实现了基于光谱自编码微球的多样品免疫荧光混合筛选和荧光成像。

Effect of bead sizes,temperatures,pH and mass concentrations of coating solution on immunosorption of microbeads was studied.20 encoding beads were coated with 20 samples correspondingly.All beads were mixed for multi-samples immunofluorescence mix-screening.The sample having positive reaction with assay was defined by identifying IR spectrum of the bead with strongest fluorescence signal.The volume dilution rate of FITC assay in screening can reach 1∶2000.Applying Ar^+-laser fluorescence scan-imaging technology,immunofluorescence mix-screening of multi-samples and fluorescence imaging based on optical self-encoding microbeads was realized successfully.