人体19种氨基酸单体的核磁共振波谱特征信息表达

CHARACTERISTICS OF NUCLEAR MAGNETIC RESONANCE HYDROGEN SPECTRUM OF MONOMERS OF HUMAN 19 AMINO ACIDS

目的:随着生物医学迅猛的发展,更多的新技术新方法被引入实验室,并逐步应用于人体超微结构与功能的研究,其中多肽、氨基酸单体的生物活性研究愈加受到有关课题的重视。并且多学科的渗透和多技术的交叉已形成新的模式,鉴定其结构的性质分析手段亦跃升到借助物理化学等高新技术,通过物质本身官能团的多组信息进行特征分析。方法:根据构成蛋白质骨架的主要原素——氢具有产生磁矩、有自旋能力的特性,采用Nuclear magnetic resonance—NMR核磁共振(氢)波谱技术,对L-CLY等19种氨基酸进行了研究。结果:15种氨基酸的波谱特征表达与国外相关研究十分吻合,并且首次获得L.D—His和L.D—Trp4种氨基酸的结构信息。结论:本组研究设立HMR分析条件:脉宽PW=5.8,增益RG=26,驰豫=0.2.采样NS=36,谱宽SW=4000Hz,偏置01=3800条。实验证明能满足分析需要。

Hydrogen is a main element to buildup proteins. According to its characters of self- rotation and magnetic moment, the hydrogen spectrum of 19 amino were studied by nuclear magnetic resonance (NMR) method. The detected spectrum features of 15 aminos were coordinated with foreign re-portedof 4 acid aminos. i.e. L. D. -His and L. D- Trp. The NMR analytic conditions were: PW = 5.8. RG = 26. NS=36, SW = 400()HZ, RO=0. 2. OL = 3800.