胶原蛋白温度效应的Raman光谱研究

Temperature Dependence of Collagen by Raman Spectra

在不同温度下对Ⅰ型胶原蛋白进行了拉曼光谱测定。结果表明随温度的升高,多数谱线向低波数移动,但CH2的变形振动谱线1302cm-1向高波数移动,苯丙氨酸的特征振动谱线1003cm-1的波数基本保持不变。同时还给出了拉曼谱线强度随温度的变化关系,得到了0,40,68,90℃四个变性峰,其中40,68℃的峰与DSC(差示扫描量热法)和SHG(二次谐波法)的测量结果一致,0℃的峰为冰冻相变,90℃的变性峰为胶原的二级结构被破坏所致。当温度达到150℃时,谱线强度显著降低,大部分谱线消失,胶原的一级结构遭到破坏。实验还发现胶原纤维在低温区具有较好的复性特性。

Raman spectra of collagen I at different temperature were obtained. There was no change at 1003 cm(-1) line, 1302 cm(-1) line moved to a higher wave number, but other lines moved to lower wave number when temperature increased. In addition, the authors observed the temperature dependence of Raman intensity and four denaturation points at 0, 40, 68 and 90 degreesC respectively. The points at 40 and 68 degreesC are in agreement with the experimental data by DSC and SHG. The point at 0 degreesC might be frozen transition; the point at 90 degreesC might be related to the damage of secondary structure. When heated to 150 degreesC, the Raman intensity of all bands decreased rapidly and many lines disappeared.