摘要
通过荧光分析方法,比较了20%SDS(Wt%),15%TritonX100(φ为体积百分比V/V)及DMF等不同溶剂对K562细胞内摄取的两亲性酞菁锌ZnPcS2P2提取效率,结果表明TritonX100具有较高的一次提取率。以15%TritonX100为提取剂,获得了K562细胞摄取ZnPcS2P2及ZnPcS2P2低密度脂蛋白(LDL)复合物的动力学曲线。结果显示,K562细胞对ZnPcS2P2LDL的最大摄取量是ZnPcS2P2的两倍,表明LDL是有效的靶向载体。
The di-sulfonated di-phthalimidomethyl phthalocyanine zinc(ZnPcS2P2) was an amphiphilic photosensitizer for photodynamic therapy of cancer. According to fluorescence analytical method, extracting efficiencies of 2.0% SDS(Wt%), 1.5% Triton X-100 (phi) and DMF for ZnPcS2P2 in K562 cells were compared. The results showed that 1.5% Triton X-100 was the most efficient reagent for the first time extracting. Using 1.5% Triton X-100 as extracting reagent, the kinetic curves of cellular uptake of ZnPcS2P2 as well as LDL-ZnPc2P2 complex by K562 cells were determined. Compared with ZnPcS2P2, the maximum cellular uptake of LDL-ZnPcS2P2 by K562 was doubled, which indicated that LDL was an effective targeting delivery vehicle for ZnPcS2P2.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2004年第11期1415-1418,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金青年基金(20201005)
教育部高等学校骨干教师资助计划
福建省自然科学基金(C010005)资助项目