电热蒸发ICP-AES法直接测定人血清中微量元素

Direct Determination of Trace Elements in Human Serum by Electrothermal Vaporization ICP-AES

建立了悬浮体制样电热蒸发电感耦合等离子体原子发射光谱法(ETVICPAES)直接测定人血清中微量元素(Cu,Cr,Fe)的方法。采用聚四氟乙烯(PTFE)悬浮体为化学改进剂,以促进待测物的蒸发,改善方法的分析性能。研究了影响待测元素信号强度的主要因素。在优化实验条件下,方法的检出限为14ng·mL-1(Cu)和29ng·mL-1(Fe),相对标准偏差小于60%。

A method for direct determination of trace elements in human serum by electrothermal vaporization inductively coupled plasma atomic emission spectrometry (ETV-ICP-AES) with slurry samplimg was developed. A polytetrafluoroethylene (PTFE) emulsion was used as a fluorinating agent to promote the vaporization of the analytes from graphite furnace, resulting in a remarkable improvement of analytical performances for the recommended method. The main factors affecting emission signals of the interested elements were investigated. The detection limits of 1.4 ng(.)mL(-1) for Cu and 2.9 ng(.)mL(-1) for Fe were achieved, and the precisions expressed as relative standard deviation (RSD) were less than 6.0% after optimization of the operating conditions.