HPLC法测定抗感口服液中麻黄碱含量

Determination of ephedrine hydrochloride in Kanggan Oral Liquor by HPLC

目的:建立抗感口服液中盐酸麻黄碱的含量测定方法。方法:采用高效液相色谱法,以C_(18)为固定相,流动相为磷酸二氢钠0.25mol/L(用磷酸调节 PH=3.5)水溶液-甲醇(70∶30),检测波长为214nm。结果:盐酸麻黄碱进样量在0～0.576mg/ml 范围内与峰面积有良好的线性关系,r=0.9990,平均回收率 RSD=0.31%。结论:本法专属性强、重现性好。

Objective:To establish a HPLC method for determination of ephedrine hydrochlo- ride in Kanggan Oral Liquor.Methods:A C18 column was used.The mobile phase consisted of NaH_2PO_4 0.25mol/L(use H_3PO_4 to make PH=3.5)-CH_3OH(70:30) and detection wavelength was 214nm.Results.The calibration curve was linear between 0～0.576mg/ml,r= 0.9990,the av- erage recovery rate was RSD= 0.31%.Conclusion:This method was reproducible and was suit- able for the quality control of Kanggan Oral Liquor.