薄层色谱法分析Mo(Ⅵ)-氨基酸配合物

Separation and Identification of Mo ( Ⅵ )-AminoAcid Coordination Compounds by Thin-Layer Chromatography

我们曾合成了两种新的Mo(Ⅵ)-氨基酸配合物:Mo(Ⅵ)-甘氨酸和Mo(Ⅵ)-胱氨酸,它们是研究钼酶的模型物。通过对它们性质的认识,有助于揭示钼酶结构上的特点及其生理功能特点,有助于加深对铝酶催化机理的了解。Stahl曾研究过无机钼化合物的薄层色谱法,经试验,不适于钼-氨基酸配合物的分析。陆熙炎等曾建立了该类配合物的纸色谱和柱色谱的鉴别和分离方法。关于钼(Ⅵ)-氨基酸配合物的薄层色谱分析法尚未见报道。为此,我们开展了研究,得到了满意结果。

A thin-layer chromatographic procedure has been developed for the separation and identification of Mo ( Ⅵ )-amino acid coordination compounds which were separated on silica gel GF plate with methanol -ethanol (7 :3)(pH=3) as the developer, and detected at 254nm. The limits of detection were lower than 4μg. The Rf-values of Mo ( Ⅵ )-amino acid coordination compounds were also determined.