高效液相色谱法测定氨苄青霉素的含量

Quantitative Determination of Ampicillin by High Performance Liquid Chromatography

氨苄青霉素及钠盐的含量测定方法有硝酸汞-电位滴定法、生物法、剩余碘量法。最新版的美国药典改用高效液相色谱法(HPLC)。现多采用咪唑汞-分光光度法,但该法稀释步骤多,又需准确控制加试剂的量、pH值\,水浴温度和时间,精密度差,费时费物。本文采用反相HPLC法,以咖啡因为内标,用峰面积比值对氨苄青霉素进行定量测定,结果令人满意。

Ampicillin content was determined by high performance liquid chromatography using an analytical column (30×0. 46cm i. d. ) packed with Spherisorb-C18(10μm) and a UV detector at 230nm and 0.01 mol/L sodium acetate (pH 5. 0)-acetonitrile (90: 10) as eluent. The quantitative determination was performed with caffeine as the internal standard. The method is satisfactory for the quality control of ampi-cillin.