应用近红外透射光谱法测定水稻种子直链淀粉含量的初步研究

Application of Near Infrared Transmittance Spectroscopy (NITS) to the Determination of Amylose Content of Rice Grain

以124份水稻种子为样本,利用FOSS-Tecator公司的Infratec1255型近红外谷物品质分析仪,对样本进行光谱扫描,并利用化学法测定了直链淀粉含量。借助于近红外定标软件(WinISI),采用多种计量数学处理方法和不同的回归统计方法进行定标曲线的开发和比较,得到了小样本量水稻种子直链淀粉含量测定的近红外分析定标数学模型。其定标标准偏差(SEC)、交叉检验标准误差(SECV)和定标决定系数(RSQ)分别为1.489、1.761和0.885。

One hundred and twenty-four samples of small amount of rice grain were scanned by Foss Tecator Infratec 1255, and their amylose contents were determined by chemistry method. With the WINISI calibration maker software, several mathematics treatments to the sample spectra and several different regression methods were adopted to optimize a good calibration equation for determination of amylose content in small amount of rice grain. The standard error of calibration (SEC), standard error of cross-validation (SECV) and regression squared (RSQ) of the calibration selected were 1.489, 1.761 and 0.885.