摘要
金霉素属于四环素类抗生素,在金霉素的生产和贮存期间产生一些四环素类的副产物,有的有一定药效,有的是有害物质。目前我国对金霉素的检验采用微生物效价测定法和薄层色谱法。国外近年内有用高效液相色谱法,但至今未见公开报道。经过一段时间的摸索,我们建立了高效液相色谱法测定金霉素及其杂质的方法,并发现了以前未知的杂质。
An HPLC procedure for the determination of chlortetracycline and impurities was developed. The column was packed with μ-Bondapak C18 and operated at 35 ℃- The detection wavelength was 350 nm. The mobile phase was 0. 1mol/L citric acid (pH = 2.8) : DMF : CH3CN = 80 : 15 : 5. The quantitative analysis was performed by external standardization. By regression analysis for the calibration curve, the correlation coefficients obtained for chlortetracycline, 4-epichlortetracycline and tetra-cycline were 0.9998, 0. 9997 and 0. 9989, respectively. This method is fast, accurate, specific and suitable for routine analysis.
出处
《色谱》
CAS
CSCD
北大核心
1993年第2期114-115,共2页
Chinese Journal of Chromatography
