高效液相色谱法测定橙汁中L-抗坏血酸

Measuring L -Ascorbic Acid in Orange Juice by Reversed-Phase High Performance Liquid Chroma- tography (HPLC)

测定橙汁中L-抗坏血酸的含量对于柑桔类产品的加工具有极为重要的意义。常见的测定方法有滴定法、苯肼法、荧光法、紫外直接测定法,此外尚有电位滴定和库仑滴定法等。但这些方法都存在一定的缺点,不是易受还原物质干扰,就是费工费时,缺乏稳定性。本文方法以高效液相色谱法(HPLC)为参考,克服了原方法中流动相离子强度过大从而出现系统峰并降低固定相使用寿命的缺点,具有准确、快速、方便和流动相价廉之优点,是一种测定L-抗坏血酸(L-AsA)的可行方法。

A reversed-phasc HPLC method was developed for the determination of L-ascorbic acid in orange juice. Under the chromatographic conditions selected by this paper, the determination for L -AsA is favorable at lower ionic strength, lower pH and lower concentration of metaphosphoric acid. Average recovery and variation coefficient were 102. 5% and 2. 23%, respectively.