高效液相色谱法测定食品中有机酸

Determination of Oganic Acids in Foods by High Performance Liquid Chromatographv

前言 有机酸是食品重要的组成成分之一,它与食品加工、贮存、质量评价等密切相关,因此越来越受到人们的重视。近年来,高效液相色谱(多采用离子色谱和离子交换色谱)已广泛应用于食品中有机酸的分析。 本文采用反相色谱,以0.6％KH_2PO_4(pH 2.65)为流动相,测定了酱油、黄酒、豆奶中的八种有机酸,取得了很好的分离效能。此法具有灵敏度高、重现性和回收率好、流动相价格便宜等特点。

This paper describes a new method for the determination of formic, acetic, lactic, malic, succinic, citric, propionic and butyric acids by high performance liquid chromatography. The recoveries were from 90. 32% to 109. 1%. This method is simple, selective and sensitive.