摘要
(二)样品预处理 稗草样品经溶剂萃取浓缩后,上1.5cm×20cm硅胶H干柱,以苯-乙酸乙酯-乙酸(50∶3∶1)混合液洗脱。洗脱液分步收集,经以苯-乙酸乙酯-乙酸(30∶5∶2)为展开剂的高效薄层检测,254nm紫外光显迹检查,合并富含R_f≈0.74斑点。
Five groups of suitable developing solvents of silica gel TLC for separating and identifying the ABA in Barnyard grass are reported. They are toluene-ethyl acetate-acetic acid glacial ( 40 : 5 : 2 ). benzene-ethyl acetate-glacial acetic acid (30 : 5: 2), 2-propanol-ammonia solution-water (10 : 1: 1) , ben-zene-n-butanol-glacial acetic acid (80 : 15 : 5) and methanol-chloroform(1: 10).
出处
《色谱》
CAS
CSCD
北大核心
1993年第6期388-388,360,共2页
Chinese Journal of Chromatography