几种适宜于稗草中脱落酸薄层色谱分离和鉴定的展开剂

The Suitable Developing Solvents of Silica Gel Thin-Layer Chromatography (TLC) for Separating and Identifying Abscisic Acid (ABA) in Barnyard Grass

(二)样品预处理 稗草样品经溶剂萃取浓缩后,上1.5cm×20cm硅胶H干柱,以苯-乙酸乙酯-乙酸(50∶3∶1)混合液洗脱。洗脱液分步收集,经以苯-乙酸乙酯-乙酸(30∶5∶2)为展开剂的高效薄层检测,254nm紫外光显迹检查,合并富含R_f≈0.74斑点。

Five groups of suitable developing solvents of silica gel TLC for separating and identifying the ABA in Barnyard grass are reported. They are toluene-ethyl acetate-acetic acid glacial ( 40 : 5 : 2 ). benzene-ethyl acetate-glacial acetic acid (30 : 5: 2), 2-propanol-ammonia solution-water (10 : 1: 1) , ben-zene-n-butanol-glacial acetic acid (80 : 15 : 5) and methanol-chloroform(1: 10).