荧光蛋白标记的促红细胞生长素融合蛋白的设计和预测

The design and prediction of hEPO fusion protein marked with EGFP protein and its biology characteristics

目的 :探讨pTRE顺式作用元件调控 ,EGFP标记hEPO(hEPO EGFP)融合蛋白设计的合理性。方法 :应用GeneConstructionKit2 5、www .expasy .com网站提供的分析方案 ,分析重组体的开放读框以及融合蛋白的柔性 ,并作蛋白质二级结构模拟。结果 :重组体的转录受pTRE顺式作用元件调控 ,Linker所在部位柔性高 ,融合蛋白表达后 ,二级结构预测Linker不改变蛋白结构 ,完全符合作者设计EGFP标记hEPO融合蛋白的初衷。结论 :重组蛋白设计合理 ,融合蛋白有很大可能保留了hEPO和EGFP的理化特性 ,为进一步的实践操作提供了可靠的理论基础。

Objective To design hEPO fusion protein marked with EGFP protein. Methods Using the sequence analysis software and protocols prescribed by website (www.expasy.com), the open reading frame of the recombinant and the flexibility of hEPO fusion protein were analyzed, and the secondary structure of hEPO linker EGFP fusion protein was simulated. Results The transcription of the recombinant protein was regulated by the cisacting element of pTRE. The fusion protein has correct domains of hEPO and EGFP. Linker has high flexibility and does not influence the secondary structure of fusion protein. The designed hEPO marked with EGFP protein was after our own heart. Conclusion The results of computer analysis could help to rationally design the recombinant protein. The attenuated hEPO and EGFP protein keep their maximum biological activities and provide a reliable theoretical basis for further research.