铜负荷饲养兔肝、肾组织含铜量和组织病理学观察

Copper Quantity and Pathology in Liver and Kidney of Rabbits Fed with Copper loading Dietary

探讨了新西兰兔在不同剂量铜负荷饮食时肝、肾组织含铜量和组织病理的改变。30只新西兰兔分为5组,对照组(第1组)给与正常饲料,每天每公斤体重120g,铜负荷组(第2、3、4、5组)给予含铜的饲料,铜剂量分别为每天每公斤体重01、1、10、20mg,其余饲料用正常饲料补足。每周测体重1次,每10d抽空腹静脉血1次查血清谷丙转氨酶(ALT)及血清铜,每半个月肝穿刺肝组织查铜一次,并做肝组织HE染色光镜下检查。实验结束时,耳静脉注射空气处死动物,取肝、肾组织块作病理检查及组织铜离子含量测定。5个月饲养期内,实验组血清ALT及血清铜离子含量与对照组相比差异无显著性,但组织中的铜离子含量随饲料含铜量的增加而增加。实验组肝组织铜离子含量与对照组相比差异有显著性(P<005),其中最大剂量组与对照组相比差异有极显著性(P<0001)。肾组织铜离子含量中,第4、5组与对照组相比差异有显著性(P<005)。病理检查中,第2、3组肝脏病理改变轻微,第4、5组有较明显的坏死病变。作者认为新西兰兔对铜的耐受性较好,较大剂量、较长时间服用有较好的安全性,可用于铜实验动物模型的建立,但进食剂量过大(含铜20mgkg体重·d以上),短期内可引起严重的肝肾器官病变。

To investigate the effect of overloaded dietary copper on New Zealand rabbits′ weight, serum alamine aminotransferase and copper concentration, as well as pathology of some organs, 30 rabbits were divided into 5 groups. The control group was fed with normal diet, and the experimental groups were given copper loaded diet at 0 1?mg, 1?mg, 10?mg, and 20?mg per kilogram body weight per day. The rabbits were weighted every week, blood was taken every ten days to measure serum alamine aminotransferase and copper concentration, and liver biopsy was conducted to for copper detection and pathological observation. At the end of experiment, all rabbits were killed by injecting air through the ear vein. The tissues of their liver and kidney were taken for pathological onservation and copper detection. All data were analyzed by SPSS. There was no statistical significance in serum alamine aminotransferase and copper concentration between experimental groups and control group during the course of the experiment, but copper concentration in the tissue was increased with the increased diet copper loading. The copper concentrations of liver and kidney increased significantly compared to the controls. There were not distinct pathological changes in control rabbits and low copper dietary loading groups, but with the copper loading increased, the necrosis in liver was evident. The damages were obvious in kidney of rabbits that were fed with highest dosage copper. Thus, we suppose that New Zealand rabbits have a good endurance to copper loading dietary. They can be used to establish an animal model of copper dietary overload.