热应激蛋白70基因多态性与急性高原反应的关系

Relationship between heat stress protein 70 gene polymorphisms and the risk of acute mountain sickness

目的 探讨热应激蛋白 (HSP) 70 1和HSP70 2基因多态性与急性高原反应的关系。方法 以在进藏训练中出现急性高原反应的 5 6名战士作为研究病例 ,以未出现急性高原反应的 173名战士作为对照 ,应用聚合酶链式反应 -限制性片段长度多态性 (PCR RFLP)方法分析了这些战士HSP70 1、HSP70 2的基因多态性。结果 HSP70 1的基因型和等位基因频率在两组间的差异无显著性(P >0 .0 5 ) ;而病例组HSP70 2B/B基因型频率 (2 3.2 % )明显高于对照组 (6 .9% ) ,差异有显著性 (P <0 .0 5 ) ,比值比为 4 .0 2。结论 携带HSP70 2B/B基因型的机体应激能力较弱 ,易发生急性高原反应 ,这将有助于在特定职业人群中发现和保护这些个体 ,为保障健康、提高工效提供科学依据。

Objective To investigate the relationship between heat stress proteins 70(HSPs70) gene polymorphism and the risk of acute mountain sickness. Methods Fifty six soldiers with acute mountain sickness and 173 soldiers without that were chosen as cases and controls.HSP70 1,HSP70 2 genotypes were analyzed by using the polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) technique. Results The HSP70 1 polymorphism was similar in two groups.The genotype frequency of HSP70 2 B/B in acute mountain sickness group(23.2%) was significantly higher than that in the control(6.9%, P <0.05, OR =4.02). Conclusion There is a significantly increased association of HSP70 2 B/B genotype with the risk of acute mountain sickness.Individuals with HSP70 2 B/B genotype may have weaker adaptive ability than those without this genotype under altitude stress.The results contribute to provide scientific bases for finding these individuals in specified occupational people,ensuring their health and enhancing work efficiency.