药物干预对大鼠急性心肌梗死组织肾素-血管紧张素系统的影响

Effect of intervention on renin-agiotensin system after acute myocardial infarction in rats

目的 :观察氯沙坦、培多普利对大鼠急性心肌梗死 (AMI)后组织肾素 血管紧张素系统 (RAS)的影响。方法 :结扎左冠状动脉制作AMI模型 ,随机分为对照组 (C组 ) ,氯沙坦组 (L组 ) ,培多普利组 (P组 ) ,氯沙坦加培多普利组 (LP组 ) ,假手术组 (S组 )。 3d、1和 6周后观察心脏组织血管紧张素Ⅱ (AngⅡ )、血管紧张素转化酶 (ACE)、左心室梗死区和非梗死区AngⅡ 1型受体 (AT1R)和AT2 RmRNA表达的变化。结果 :①C组和L组非梗死区心肌组织AngⅡ含量均显著高于S组 (均P <0 .0 1) ,P组和LP组非梗死区心肌组织AngⅡ含量和ACE水平均显著低于C组和L组 (P <0 .0 1)。②AT1RmRNA表达率 :术后 3d ,4个MI组梗死区均明显增高 (P <0 .0 1) ,1周时 ,非梗死区C和P组显著高于S组 (均 P <0 .0 1) ,梗死区C和P组 (P<0 .0 1)及L和LP组 (P<0 .0 1)显著增高。 6周时 ,非梗死区和梗死区C和P组显著增高 (P <0 .0 1)。③AT2 RmRNA表达率 :3d时 ,梗死区 4个MI组显著增高 (P <0 .0 5 ) ;1周和 6周 ,4个MI组非梗死区 (C组和P组 :P <0 .0 5 ;L组和LP组 :P<0 .0 1)和梗死区 (P <0 .0 1)均显著增高 ;1周时L组梗死区和非梗死区显著高于C组和P组 (P <0 .0 5 ) ;6周时L组和P组在梗死区和非梗死区显著高于C组和P组 (P <0 .0 5 )。结论 :氯沙坦。

Objective: To investigate the effect of Losartan ,Perindopril on RAS after acute myocardial infarction (AMI) in rats. Method: Animal models of AMI were made in rats and randomly divided into 5 groups: sham-operated group (S), control (C), treated with Losartan (L), Perindopril (P), and combined (LP). After 3 days, 1 and 6 weeks, Tissure Ang Ⅱ and ACE activity were assessed by radioimmunoassay(RIA) and ultraviolet spectrotometry. The AT 1 and AT 2 receptors mRNA level were quantified by reverse transcription-polymerase chain reaction (RT-PCR). Result:①the tissue Ang Ⅱ of non-infarcted myocardial portion in C and L groups significantly increased(P< 0.01), the tissue Ang Ⅱ and ACE of non-infarcted myocardial portion in P and LP groups were significant lower than that in C and L groups(P< 0.01). ② the AT1R mRNA expression: At 3 days after AMI, it increased significantly only in infarcted portions in the four MI groups(P< 0.01). At 1 weeks , it was lower in non-infarcted portion in L and LP and S groups than that in P and C groups(P< 0.01), At 6 weeks, it significantly increased in P and C groups of infarcted portion(P< 0.01) and non-infarcted portion(P< 0.01).③ the AT2R mRNA Expression: At 3 days after AMI, it had significant increase only in infarced portions in the four MI groups(P< 0.05);At 1 week, it significantly increased in infarcted and non-infarced portions in L groups(P< 0.05). And at 6 weeks, it was significant higher in infarcted and non-infarced portions in L and LP groups than that in C and P groups(P< 0.05). Conclusion: Losartan and Perindopril could improve left ventricular remodeling and heart function in AMI rats.