摘要
目的 研究TNF α与癫痫发病的关系 ,进一步探讨癫痫发病机理及为癫痫的临床治疗提供依据。方法 将SD大鼠随机分为空白对照组、生理盐水对照组和马桑内酯 (CL)致痫组 ,侧脑室给药后观察记录两组大鼠行为变化 ,存活 2h灌注取材切片 ,用SABC法进行免疫组织化学染色及图像分析观察大鼠海马内TNF α和TNFR1表达的变化。结果 CL致痫组均出现Ⅲ～Ⅴ级痫性发作 ,对照组无发作 ;CL致痫组TNF α与TNFR1在大鼠海马各区阳性细胞数较对照组增多 ,表达较对照组增强 ,其中TNF α在海马CA1、CA3区锥体细胞层 ,TNFR1在海马CA3区锥体细胞层表达显著增强 (均P <0 0 1)。结论 TNF α在癫痫发病中起促进作用 ,其作用可能是通过TNFR1介导的。
Objective To explore the relationship between epilepsy and tumo r necrosis factor-α(TNF-α). Methods The SD rats were randomly divided into three groups: th e blank control group, normal saline control group and Coraria Lactone (CL) grou p. Epilepsy was induced by injecting CL into the lateral ventricle in CL group. The behavioral changes of rats in three groups were observed. The expression of TNF-α and TNFR1 was detected by immunohistrochemistry and image analysis in hi ppocampus 2 h after injection. Results Serve epileptic behavior occurred after injection of CL . The expression of TNF-α and TNFR1 was enhanced and much more positive cells were found in hippocampus of the rats in CL group. Especially, TNF-α in CA1 an d CA3 area and TNFR1 in CA3 area in CL group were stronger than those in control groups. Conclusion TNF-α may play a promoting role in CL-induced epi lepsy probably mediated by TNFR1.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2004年第6期655-658,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
