Survivin在胃癌组织中的表达及其与caspase-3、p53基因表达相关性的初步研究

Expression of Survivin Gene and Its Rela tionship with Expression of caspase-3, p53 in Gastric Carcinomas

目的 研究Survivin在胃癌组织中的表达及其与caspase 3、p5 3基因表达的相关性。方法 收集 32例胃癌组织标本及相应的癌旁组织 ,利用免疫组织化学法检测Survivin、caspase 3、p5 3基因的表达。结果 6 2 5 % (2 0例 )的胃癌组织表达Survivin蛋白 ,而癌旁组织内无阳性表达。Survivin表达与胃癌组织的大小、分化程度、临床分期及淋巴结转移无明显相关关系。Caspase 3、p5 3蛋白与Survivin表达显著相关。结论 Survivin在胃癌组织中选择性表达 ,提示该基因在胃癌的发生发展中起重要作用。Caspase 3的失表达、p5 3的突变与Survivin基因的表达可能在胃癌发生中起协同作用。

Objective To study the expression of Survivin and its relations hip with expression of caspase-3 in gastric carcinomas. Methods Immunohistochemical assay was used to detect the expres sion of Survivin, caspase and p53 proteins. Results Survivin protein was detected in 20 of 32 (62.5 %) gas tric cancerous tissues, but no expression of Survivin was detectable in correspo nding paracancerous tissues. The expression of Survivin was not related to the s ize of tumor, differentiation, stage and lymph node metastasis. Conclusion The expression of Survivin in gastric cancerous tiss ues was up-regulated, indicating this gene may play an important role in the pr ocess of carcinogenesis of gastric cancer. The changes in the level of Survivin, caspase-3 and p53 play important roles in the pathogenesis of gastric cancer.