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运用PCR检测猪圆环病毒的研究 被引量：2

Research on Application of PCR for Detection of Porcine Circovirus
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摘要 设计和合成了一对引物,建立PCR反应,用于检测猪圆环病毒。结果表明,PCR对猪圆环病毒细胞毒,病毒基因克隆具有较好的特异性和灵敏性,提示用PCR试验检测猪圆环病毒是一种较好的方法。 A pair of primers were designed and synthesized, and a PCR was established for detection of porcine circovirus.The result showed that the PCR was very specific and sensitive to porcine circovirus and the virus gene clone,indicating that it is a good method for detection of porcine circovirus.
作者 龚振华 刘俊辉 张利友 刘国庆 黄秀梅 李葳 王玉东 郭福生 蒋正军 郑增忍
机构地区 农业部动物检疫所 山东省胶南市畜牧兽医服务中心
出处 《中国动物检疫》 CAS 北大核心 2005年第1期24-26,共3页 China Animal Health Inspection
关键词 PCR 检测 猪圆环病毒 基因克隆 Porcine circovirus,PCR
分类号 S852.659.2 [农业科学—基础兽医学]
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参考文献6

  • 1Tischer I, Mields W, Wolff D, Studies on epidemiology and pathogenicity of porcine circovirus [J]. Arch Virol. 1986,91(3 - 4) :271 - 6.
  • 2Allan G M, McNeilly F, Ellis J. Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication[J]. Arch Virol. 2000, 145(11) :2421 - 9.
  • 3Wellenberg G J, Pesch S, Bemdsen F W, Isolation and characterization of porcine circovirus type 2 from pigs showing signs of postweaning multisystemic wasting syndrome in the Netherlands[J]. Vet Q.2000,22(3) : 167- 72.
  • 4Larochelle R,Bielanski A, Muller P. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen[J] .J Clin Microbiol.2000, 38(12) : 4629 - 32.
  • 5Wellenberg G J, Pesch S, Bemdsen F W, Isolation and characterization of porcine circovirus type 2 from pigs showing signs of postweaning multisystemic wasting syndrome in the Netherlands[J]. Vet Q. 2000,22(3) : 167- 72.
  • 6Larochelle R,Bielanski A, Muller P. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen[J] .J Clin Microbiol. 2000, 38(12) : 4629 - 32.

同被引文献31

  • 1崔尚金,蔡阳,陈欣.检测猪圆环病毒DNA的竞争定量PCR方法的建立及初步应用[J].中国预防兽医学报,2004,26(5):347-351. 被引量：21
  • 2殷鹰,聂奎,杨戈,胡燕宾,易华珍.ELISA对猪场PCV-Ⅱ血清抗体的检测[J].畜牧市场,2005(8):177-179. 被引量：3
  • 3欧阳岁东,胡奇林,陈仕龙,林锋强,程小霞,朱小丽.用PCR法检测猪繁殖-呼吸综合征病毒和猪圆环病毒混合感染病例研究[J].福建畜牧兽医,2005,27(4):5-6. 被引量：3
  • 4朱骏,曹向英,沈林辉,彭笑蓉,张慧英,孙文梅.上海市松江区猪圆环病毒Ⅱ型感染的血清学调查[J].福建畜牧兽医,2006,28(1):21-22. 被引量：6
  • 5吕艳丽 杨汉春 郭鑫.猪圆环病毒2型分离毒株基因组序列测定与分析[J].中国预防兽医学报,2002,:63-66.
  • 6Tiseher I,Raseh R,Toehtermann G.Characterization of popovavirus and pieomaviruses like particles in permanent pig kidney cell lines[J].Zentralb1 Bact Microbio1 Hyg Ser A,1974,(226):153-167.
  • 7Huang C I,Hung J J,Wu C Y,et al.Multiplex PCR for rapid detection of pseudorabies virus,porcine parvovirus and porcine circoviruses[J].Veterinary Microbiology,2004,101(3):209-215.
  • 8Calsamiglia M,Segal S J,Qnintana J,et al.Detection of porcine circovirus types 1 and 2 in serum and tissue samples of pigs with and without postweaning multisysternic wasting syndrome[J].Journal of Clinical Microbiology,2002,40(5):1848-1850.
  • 9Kim J,Chae C.Multiplex nested PCR compared with in situ hybridization for the differentiation of porcine circoviruses and porcine parvovirus from pigs with postweaning multisystemic wasting syndrome[J].Can J Vet Res,2003,67(2):133-137.
  • 10Liu Q,Wang L,Willson P.Quantitative competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome[J].Journal of Clinical Microbiology,2000,38:3474-3477.

引证文献2

中国动物检疫
2005年 第1期

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