胸腺五肽一级结构的电喷雾质谱分析

Determination of the first-order structure of thymopentin(TP5)by elec trospray ionization mass spectrometry

目的测定胸腺五肽的相对分子质量及其一级结构。方法应用电喷雾质谱法(ESIMS)及源内碰撞诱导解离技术对胸腺五肽进行测定,根据其典型碎片离子确证其氨基酸序列及相对分子质量。结果测得胸腺五肽准分子离子峰(M+H)+m/z680.3及(M+Na)+m/z702.2,(M+2Na-H)+m/z724.3及(M+3Na-2H)+m/z746.2,表明胸腺五肽的相对分子质量为679.4,与理论值一致。结论胸腺五肽的相对分子质量及一级结构与理论值相符。

Objective To determine the molecular weight and the first-order structure of thymopentin. Methods The molecular weight and amino acid sequence of thymopentin were determined by electrospray ionization mass spectrametry. A series of typical fragment ions were obtained by in-source collision-induced dissociation (CID). The b series of fragment ions were obtained by adjusting the fragmentor voltage. Results The quasi-molecular ions were 680. 3(M + H)+and 702. 2(M+Na)+, 724. 3(M + 2Na-H)+, 746. 2(M + 3Na-2H) + . Conclusion The molecular weight and first-order structure of thymopentin were confirmed.