螨过敏性变应性鼻炎血清CD_(23)与sIgE的相关性研究

A study on the correlation of CD_(23) expression in lymphocytes and sIgE level in serum of cases with allergic rhinitis induced by dust mites

目的 研究螨过敏性变应性鼻炎患者细胞表面分化抗原(CD23)的表达与血清IgE的相关性。方法采用流式细胞术检测33例螨过敏性变应性鼻炎患者、20例正常对照者外周血淋巴细胞CD23分子的阳性细胞百分率,同时用免疫化学发光法检测血清IgE水平,并进行相关性分析。结果 螨过敏性变应性鼻炎患者外周血淋巴细胞CD23分子的阳性细胞百分率和血清IgE分别为(8.10±2.65)％、(16.29±3.40)μmol／L,明显高于正常对照组(5.12±1.74)％、(1.37±0.57)μmol／L;且CD23分子的阳性细胞百分率与血清IgE水平呈显著正相关。结论 螨过敏性变应性鼻炎患者淋巴细胞CD23的表达和血清IgE密切相关,在该病的发病机制中具有重要作用。

Objective To investigate the correlation of differentiating antigen CD23 in B lymphocytes and IgE level in serum of cases with allergic rhinitis (AR) induced by dust mites. Methods 33 cases with dust mites induced AR and 20 cases of healthy people were included in this study. The differentiating antigen CD23 was detected in peripheral blood B lymphocytes by flow cytometry(FCM)amd the IgE level was determined in serum by immunochemiluminescence assay among these cases. Then,an analysis was made to see the correlation of these indexes with AR induced by dust mites. Results The positive expression of CD23 in B lymphocytes was (8- 10 ±2. 65)% and IgE level in serum was(16. 29+3- 40)μmol in the patients with dust mites induced AR, significantly higher than that (being 5-12 ±1-74% and 1. 37 ± 0. 5μmol respectively ) in healthy people. Furthermore,there was a positive correlation seen in the expression of CD23 with the serum level of IgE. Conclusion The expression of differentiating antigen CD23 in peripheral blood B lymphocytes and IgE level in serum are closely related with the pathogenesis of AR induced by dust mites.