培养基对菌产微生物絮凝剂的影响研究

Study on effect of culture medium on microbial flocculant production

研究了培养基成分种类及其用量对菌产微生物絮凝剂的影响。结果表明,较高的C/N比对菌产絮凝剂有利,碳源中的蔗糖是菌株Aeromonassp.N11产絮凝剂的良好碳源,氮源以采用复合氮源为佳。培养基中加入酵母膏有利于絮凝剂的产生。NaCl能够促进所产絮凝剂的絮凝活性,碳源蔗糖和氮源脲对菌产絮凝剂的影响最大。利用正交实验得出产絮凝剂培养基的最佳配比为蔗糖20g/L,酵母膏08g/L,脲05g/L,硫酸胺05g/L,NaCl7g/L。

The effect of the medium composition and proportion on microbial flocculant production was studied. The results showed that high C/N ratio was beneficial to microbial flocculant production; carbon source sucrose was beneficial to microbial flocculant production of Aeromonas sp. N11; the complex nitrogen source was better than the independent nitrogen source; yeast extract was beneficial to microbial flocculant production; NaCl could increase the flocculation activity of the microbial flocculant produced; carbon source sucrose and nitrogen source carbamide had the maximun effect. The optimun component proportions of medium obtained according to the orthogonal experiment were: sucrose 20 g/L, yeast extract 0.8 g/L, carbamide 0.5 g/L, (NH 4) 2SO 4 0.5 g/L, NaCl 7 g/L.