抗生素对国槐愈伤组织诱导和生长的影响

Effects of antibiotics on induction and growth of Sophora japonica L. callus.

由于不同植物不同外植体对抗生素的反应不同,在进行国槐农杆菌介导的遗传转化研究之前,必须对新霉素磷酸转移酶基因(nptⅡ)有抗性的抗生素种类及其使用浓度进行探讨.结果表明,卡那霉素在浓度达到150mgL时仍然没有完全抑制国槐叶片和茎段的生长;而遗传霉素在低浓度下对国槐愈伤组织的诱导和生长的抑制作用较卡那霉素明显.随着遗传霉素浓度的增加,愈伤组织形成的频率降低,外植体的死亡率上升.当遗传霉素的浓度超过10mgL时,叶片和茎段愈伤组织的诱导和生长已完全受到抑制.另外,研究发现不同外植体对遗传霉素的敏感程度不同,茎段较强,叶片稍弱茎段的抗生素致死浓度在8～10mgL之间,叶片的抗生素致死浓度8mgL左右.

Before attempting to transform Sophora japonica L.,the authors studied the usage of types and concentrations of antibiotics that have effects on the genes for neomycin phosphotransterase.It is found that the callus is still being formatted and continues to grow when the concentration of kanamycin reaches 150 mg/L,but geneticin at low concentration will inhibit the formation and growth of Sophora japonica L.callus more effectively than kanamycin at a high concentration. With the increase of concentration of geneticin,the frequency of callus formation decreases, while the death rate of explants rises. When the concentration of geneticin amounts to 10 mg/L,the formation and growth of the stem segment callus are completely inhibited. In addition, the results show that the sensitivity of leaf and stem to geneticin is different. The stem is a little more sensitive than leaf.The lethal geneticin concentration for stem is about 8～10 mg/L,and about 8 mg/L for the leaf.